anti-mCherry NanoBeads

 AlpaLife's Anti-mCherry Agarose Beads are excellent tools for separating mCherry-fusion proteins and their binding partners from cell extracts. By coupling high affinity anti-mCherry single domain of camelid antibodies to high quality agarose beads, mCherry Agarose Beads exhibit high specificity and strong affinity to mCherry and its fusion proteins.





Applications:

1. Immunoprecipitation (IP/ Co-IP)

2. Chromatin immunoprecipitation (Ch-IP)

3. RNA immunoprecipitation Analysis (RIP)

Advantages:

Short incubation time: 5 - 30 min for most situations.

High specificity and strong affinity with monoclonal antibody conjugated.

Reliable and consistent results with small variations.

No contamination of heavy and light chains.

Stable for stringent conditions.

Can bused directly, no need to conjugating protein A/G to antibodies.


Beads Property:

Beads: 40 μm diameter cross-linked 7.5 % agarose beads

Storage Buffer: 20 % ethanol

Binding Capacity: 10ul Anti-mCherry Agarose Beads bind 2-4 μg mCherry protein

Storage:

 for 6 month


* The name "Nanobody" belongs to Ablynx

Product

Size

Code

Price

Shipping

anti-mCherry Agarose Beads

250 μL

On request 

Worldwide shipping

anti-mCherry Agarose Beads

500 μL

(20 reactions)

KTSM1302

145 USD 

Worldwide shipping



1. Question: Can I use a different Lysis Buffer with higher NaCl and NP40 concentration?
Answer: 
Yes, the nanobody we conjugated with agarose beads is stable even in harsh buffer conditions.

2. Question: Can I incubate Anti-mCherry Agarose Beads with cell lysate overnight at 4 °C to increase their binding?
Answer: Yes, but the binding efficiency between Anti-mCherry Agarose Beads and mEos after 1 h, 2 h, 3 h and overnight incubations are almost the same. So in most situations, 1 h incubation should be enough and can give good results.



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