Anti-GFP Nanobeads

   The green fluorescent protein (GFP) is a protein exhibiting bright green fluorescence when exposed to ultraviolet light and frequently used as reporters and fusion tag. AlpaLife's anti-GFP Agarose Beads are perfect tools for separating GFP-fusion proteins and their binding partners from cell extract. By coupling high-affinity anti-GFP VHH antibodies (also known as single domain of camelid antibodies, or nanobodies *) to agarose beads, GFP Beads exhibit high specificity and strong affinity to GFP and GFP-fusion proteins.

* The name "Nanobody" belongs to Ablynx



Applications:

1. Immunoprecipitation (IP)

2. Chromatin immunoprecipitation (Ch-IP)

3. RNA immunoprecipitation Analysis (RIP)

4. Mass spectrometry

5. Enzyme activity measurements






Advantages:

Short incubation time: 5 - 30 min for most situations.

High specificity and strong affinity with monoclonal antibody conjugated.

Reliable and consistent results with small variations.

No contamination of heavy and light chains.

Stable for stringent conditions.

Can be used directly, no need to conjugating protein A/G to antibodies.

Specificity:

GFP, GFPuv, eGFP, YFP

Beads Property:

Beads: 40 μm diameter cross-linked 7.5 % agarose beads

Storage Buffer: 20 % ethanol

Binding Capacity: 10 μl GFP Agarose Beads bind 3-4 μg recombinant GFP protein

Storage:

4 for 6 month

Product

Size

Code

Price

Shipping

 

anti-GFP Agarose NanoBeads

500 μL

KTSM1301

145 USD

Worldwide shipping


Order 

1. Question: Can I use GFP Agarose Beads to immunoprecipitate EGFP- or RFP-fusion proteins and their partners?
Answer: Yes, GFP Agarose Beads have been experimentally tested on GFP, EGFP and RFP tagged proteins.

2. Question: Can I use a different Lysis Buffer with higher NaCl and NP40 concentration?
Answer: Yes, the nanobody we conjugated with agarose beads is stable even in harsh buffer conditions.

3. Question: Can I incubate GFP Agarose Beads with cell lysate overnight at 4 °C to increase their binding?
Answer: Yes, but the binding efficiency between GFP Agarose Beads and GFP after 1 h, 2 h, 3 h, and overnight incubations are almost the same. Therefore, in most situations, 1 h incubation should be enough and can give good results.



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