Nanobody can be raised from an immune library or a naïve library. Immune phage display libraries constructed from camels, alpacas, llamas or sharks can generate VHHs or VNARs with high affinity and specificity. At AlpaLife, we have succeeded in generating monoclonal antibodies from alpacas, camels, and sharks.
Higher chance to get desired VHH or VNAR single domain antibody
Better affinity and specificity
Easier to acquire antibodies recognizing different antigen epitopes
Long experiment period
Phases of Nanobody development:
Currently, constructing and screening immune antibody library via phage display technology have become a major choice for the production of monoclonal antibody with high specificity and affinity. Compared with the conventional hybridoma technology, the generation of immune antibody libraries is not limited by the requirement of fusion partners, which expands the possibility to develop monoclonal antibodies from a much broader range of species.
Moreover, AlpaLife offers small- and large-scale expression of proteins in E. coli., Pichia Pastoris and further evolution services, such as nanobody labeling and nanobody humanization. Please contact us for more information.
With AlpaLife custom VHH or VNAR antibody development service, we will carry out targeted antibody through both immunization and naive phage library to guarantee the acquisition of enough and high-sequence-variety nanobodies. Furthermore, we can perform directed evolution on selected camelid or shark antibody sequence to dramatically enhance its affinity and specificity.
* The name "Nanobody" belongs to Ablynx